1wb7

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Image:1wb7.gif

1wb7, resolution 2.24Å

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IRON SUPEROXIDE DISMUTASE (FE-SOD) FROM THE HYPERTHERMOPHILE SULFOLOBUS SOLFATARICUS. CRYSTAL STRUCTURE OF THE Y41F MUTANT.

Overview

We have examined and compared the effects of mutating Y41 and H155 in the, iron superoxide dismutase (SOD) from the archaeon Sulfolobus solfataricus, (Ss). These two neighboring residues in the active site are known to have, crucial functions in structurally related SODs from different sources. The, metal analysis indicates a slightly lower iron content after either Y41F, or H155Q replacement, without any significant substitution of iron for, manganese. The specific activity of SsSOD referred to the iron content is, 17-fold reduced in the Y41F mutant, whereas it is less than 2-fold reduced, by the H155Q mutation. The noticeable pH dependence of the activity of, SsSOD and H155Q-SsSOD, due to the ionization of Y41 (pK 8.4), is lost in, Y41F-SsSOD. After H155Q and even more after the Y41F ... [(full description)]

About this Structure

1WB7 is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with FE as [ligand]. Active as [Superoxide dismutase], with EC number [1.15.1.1]. Structure known Active Site: FEA. Full crystallographic information is available from [OCA].

Reference

The role of Tyr41 and His155 in the functional properties of superoxide dismutase from the archaeon Sulfolobus solfataricus., Gogliettino MA, Tanfani F, Scire A, Ursby T, Adinolfi BS, Cacciamani T, De Vendittis E, Biochemistry. 2004 Mar 2;43(8):2199-208. PMID:14979716

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