2pt7
From Proteopedia
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Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Overview
Helicobacter pylori is one of the world's most successful human pathogens, causing gastric ulcers and cancers. A key virulence factor of H. pylori is, the Cag pathogenicity island, which encodes a type IV secretion system., HP0525 is an essential component of the Cag system and acts as an inner, membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the, N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal, structure of HP0525 in complex with a fragment of HP1451, a protein of, previously unknown function, is reported. The HP1451 construct consists of, two domains similar to nucleic acid-binding domains. Two HP1451 molecules, bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in, the closed form and forming a partial lid over the HP0525 chamber. From, the structure, it is suggested that HP1451 acts as an inhibitory factor of, HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by, results of in vitro ATPase assay and in vivo pull-down experiments.
About this Structure
2PT7 is a Protein complex structure of sequences from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:17972918
Page seeded by OCA on Wed Jan 23 11:40:11 2008
