2r9a
From Proteopedia
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Crystal structure of human XLF
Overview
DNA double-strand breaks represent one of the most severe forms of DNA, damage in mammalian cells. One pathway for repairing these breaks occurs, via nonhomologous end-joining (NHEJ) and depends on XRCC4, LigaseIV, and, Cernunnos, also called XLF. Although XLF stimulates XRCC4/LigaseIV to, ligate mismatched and noncohesive DNA ends, the mechanistic basis for this, function remains unclear. Here we report the structure of a partially, functional 224 residue N-terminal fragment of human XLF. Despite only weak, sequence similarity, XLF(1-170) shares structural homology with, XRCC4(1-159). However, unlike the highly extended 130 A helical domain, observed in XRCC4, XLF adopts a more compact, folded helical C-terminal, region involving two turns and a twist, wrapping back to the structurally, conserved N terminus. Mutational analysis of XLF and XRCC4 reveals a, potential interaction interface, suggesting a mechanism for how XLF, stimulates the ligation of mismatched ends.
About this Structure
2R9A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal Structure of Human XLF: A Twist in Nonhomologous DNA End-Joining., Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS, Mol Cell. 2007 Dec 28;28(6):1093-101. PMID:18158905
Page seeded by OCA on Wed Jan 23 11:20:03 2008
