Luciferase

Luciferase (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, i.e. the crystal structure of firefly Luciferase (3iep). See detailed analysis of Luciola cruciata luciferase and Luciferase FMN complex- Vibrio harveyi.

3D Structures of Luciferase

Update February 2013

3iep, 1lci – fLuc – firefly

3ier – fLuc+PEG 400
1ba3 – fLuc+bromoform
3ies, 3rix, 4e5d – fLuc+inhibitor
4g36 – fLUc + dehydroluciferyl-sulfamoyl adenosine
4g37 – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine
2d1q – LcLuc (mutant)+MgATP – Luciola cruciata
2d1r - LcLuc (mutant)+oxyluciferin+AMP
2d1s, 2d1t - LcLuc (mutant)+intermediate analog
3qya – Luc (mutant) – Lampyris turkestanicus
3fgc – VhLuc α+β chain+FMN – Vibrio harveyi
1luc, 1brl - VhLuc α+β chain
1bsl, 1xkj - VhLuc β chain
2psd, 2psh, 2psj, 2pse, 2psf – Luc (mutant) – Renilla reniformis

Additional information

(2d1s)

Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

• Luciola cruciata luciferase (Japanese firefly), complexed with Oxyluciferin & AMP (2d1r) or with an high-energy analogue (2d1s)
• 3ies,3iep and 3ier Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124^[1]
• 1ba3 firefly luciferase enzyme from Photinus pyralis
• 2psd, 2pse, 2psf, 2psh, 2psj, and 2rh7 the luciferase from Renilla reniformis (RLuc)
• 1bsl 1.95Å heterodimer of bacterial luciferase from Vibrio harveyi
• 1brl 2.4Å heterodimer of bacterial luciferase from Vibrio harveyi
• 1xkj homodimer of bacterial luciferase from Vibrio harveyi
• 2d1q, 2d1r and 2d1s thermostable Japanese Firefly (Luciola cruciata) Luciferase complexed with High-energy intermediate analog
• 2d1t thermostable Japanese Firefly Luciferase red-color emission S286N mutant
• 1lci firefly luciferase enzyme from Photinus pyralis
• Dinoflagellate luciferase
• 1luc The 1.5Å resolution crystal structure of bacterial luciferase from Vibrio harveyi
• 3fgc Bacterial luciferase from Vibrio harveyi^[2]
• 1vpr luciferase of Lingulodinium polyedrum, a marine bioluminescent dinoflagellate
• Green Fluorescent Protein
• For additional information, see: Colored & Bioluminescent Proteins