399d

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STRUCTURE OF D(CGCCCGCGGGCG)

Overview

We have determined the structure of the dodecamer duplex d(CGCCCGCGGGCG)2., A careful use of the molecular replacement programme AMoRe has been, essential in order to solve the structure. This dodecamer shows a unique, conformation, quite different from all the previously studied, oligonucleotide duplexes: the central octamer has an A conformation, but, with a sharp 65 degrees kink in the centre; the terminal base-steps have a, B-like conformation; the major groove is completely closed in the centre, a hollow molecule is thus found. The results obtained confirm the high, degree of variability of DNA structure. A new type of kink and an, intermediate A/B double-helical conformation have been found. Such, intermediate conformation differs from those described in DNA polymerase, complexes.

About this Structure

399D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of the d(CGCCCGCGGGCG) dodecamer: a kinked A-DNA molecule showing some B-DNA features., Malinina L, Fernandez LG, Huynh-Dinh T, Subirana JA, J Mol Biol. 1999 Jan 29;285(4):1679-90. PMID:9917405

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