446d
From Proteopedia
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STRUCTURE OF THE OLIGONUCLEOTIDE D(CGTATATACG) AS A SITE SPECIFIC COMPLEX WITH NICKEL IONS
Overview
In this paper we explore the application of Ni2+to the crystallization of, oligonucleotides. We have determined in this way the structure of a fully, alternating (Y-R) decanucleotide d(CGTATATACG) by single crystal X-ray, diffraction. This is the first oligonucleotide crystal structure with an, alternating 5'-(TA)3-3' central part. Alternating oligonucleotides have a, particular interest since they often have a unique structure. In this case, the general conformation is B-like with an alternating twist and an, end-to-end interaction which involves terminal guanines. The crystal, belongs to space group P41212 with a = b = 52.46, c = 101.49 A. This, packing imposes a 90 degrees crossing of the symmetry related helices., This is a new way of packing for decamers.The oligonucleotide structure is, characterized by the specific association with seven nickel ions, involving the N7 atom of every guanine. One of the Ni2+ions is shared, between two guanines of symmetry related molecules. Until now no, oligonucleotide has been crystallized in the presence of this metal ion. A, novel C.A.T triplet structure has also been tentatively identified.
About this Structure
446D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the oligonucleotide d(CGTATATACG) as a site-specific complex with nickel ions., Abrescia NG, Malinina L, Fernandez LG, Huynh-Dinh T, Neidle S, Subirana JA, Nucleic Acids Res. 1999 Apr 1;27(7):1593-9. PMID:10075989
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