4fxc

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spinqualitylabelsall models 4fxc, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

TERTIARY STRUCTURE OF [2FE-2S] FERREDOXIN FROM SPIRULINA PLATENSIS REFINED AT 2.5 ANGSTROMS RESOLUTION: STRUCTURAL COMPARISONS OF PLANT-TYPE FERREDOXINS AND AN ELECTROSTATIC POTENTIAL ANALYSIS

Overview

The structure of plant-type [2Fe-2S] ferredoxin isolated from Spirulina, platensis has been refined using diffraction data to 2.5 A resolution by, alternate cycles of simulated annealing and manual revision of the model., The final R factor is 19.9% for 2,912 reflections with F > 2 sigma F, between 8.0 and 2.5 A resolution. S. platensis ferredoxin, like other, plant-type [2Fe-2S] ferredoxins, has a major alpha-helix flanking a sheet, consisting of four beta strands. The present refinement revises the, conformation of residues 56-71, in which a one-turn helix was identified., Superposition of the Spirulina ferredoxin structure on the structures of, other ferredoxins that have been well refined showed structural, perturbation at a few residues on the amino and carboxyl termini and the, turn between the first and second beta-strands. The root-mean-square, deviations of the corresponding C alpha atoms of the pairs of ferredoxins, range from 0.90 to 1.17 A for all the residues, but from 0.64 to 0.70 A if, the few perturbed residues are excluded. Therefore, it may be concluded, that the main-chain foldings of all the plant-type [2Fe-2S] ferredoxins, are essentially the same. Electrostatic potential analysis showed that the, molecular surface around the cluster is negatively charged, whereas that, of the beta-sheet of the other side is positively charged. The interaction, between ferredoxin and ferredoxin-NADP+ reductase is discussed on the, basis of the charge distributions of these molecules and biochemical data.

About this Structure

4FXC is a Single protein structure of sequence from Arthrospira platensis with FES as ligand. This structure superseeds the now removed PDB entries 3FXC and 1FXC. Full crystallographic information is available from OCA.

Reference

Tertiary structure of [2Fe-2S] ferredoxin from Spirulina platensis refined at 2.5 A resolution: structural comparisons of plant-type ferredoxins and an electrostatic potential analysis., Fukuyama K, Ueki N, Nakamura H, Tsukihara T, Matsubara H, J Biochem (Tokyo). 1995 May;117(5):1017-23. PMID:8586613

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