5mht
From Proteopedia
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TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY
Overview
The crystal structure of HhaI methyltransferase complexed with, non-palindromic duplex DNA, containing a hemimethylated recognition, sequence, and with the cofactor analog S-adenosyl-L-homocysteine (AdoHcy), has been determined. The structure provides an explanation for the, stronger affinities of DNA methyltransferases for hemimethylated DNA than, for unmethylated or fully methylated DNA in the presence of AdoHcy. The, unmethylated target 2'-deoxycytidine flips out of the DNA helix and the CH, group at position 5 makes van der Waals' contacts with the sulfur atom of, AdoHcy. Selectivity/preference for hemimethylated over fully methylated, DNA may thus reflect interactions among the chemical substituent (H or, CH3) at the C5 position of the flipped cytosine, protein and the bound, AdoHcy. The 5-methyl-2'-deoxycytidine on the complementary strand remains, in the DNA helix, with the methyl group almost perpendicular to the, carboxylate group of Glu239, which is part of the sequence recognition, loop. Thus, selectivity/preference for hemimethylated over unmethylated, DNA appears to result largely from van der Waals' contacts between the, planar Glu239 carboxylate and the methyl group of the, 5-methyl-2'-deoxycytidine. Furthermore, the positive electrostatic, potential originating from the bound AdoHcy extends to the DNA phosphate, groups flanking the flipped cytosine. The increased binding to DNA by, long-range electrostatic interactions should also occur with the methyl, donor S-adenosyl-L-methionine.
About this Structure
5MHT is a Single protein structure of sequence from Haemophilus haemolyticus with SAH as ligand. Active as Nicotinate N-methyltransferase, with EC number 2.1.1.7 Full crystallographic information is available from OCA.
Reference
A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase., O'Gara M, Roberts RJ, Cheng X, J Mol Biol. 1996 Nov 8;263(4):597-606. PMID:8918941
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