Structural highlights
4n2p is a 4 chain structure with sequence from Pyrho. Full crystallographic information is available from OCA.
Ligands: , ,
NonStd Res:
Activity: Glucokinase, with EC number 2.7.1.2
Resources: FirstGlance, OCA, RCSB, PDBsum
Publication Abstract from PubMed
Archease is a 16-kDa protein that is conserved in all three domains of life. In diverse bacteria and archaea, the genes encoding Archease and the tRNA ligase RtcB are localized into an operon. Here we provide a rationale for this operon organization by showing that Archease and RtcB from Pyrococcus horikoshii function in tandem, with Archease altering the catalytic properties of the RNA ligase. RtcB catalyzes the GTP and Mn(II)-dependent joining of either 2',3'-cyclic phosphate or 3'-phosphate termini to 5'-hydroxyl termini. We find that catalytic concentrations of Archease are sufficient to activate RtcB, and that Archease accelerates both the RNA 3'-P guanylylation and ligation steps. In addition, we show that Archease can alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently. Moreover, RtcB variants that have inactivating substitutions in the guanine-binding pocket can be rescued by the addition of Archease. We also present a 1.4 A-resolution crystal structure of P. horikoshii Archease that reveals a metal-binding site consisting of conserved carboxylates located at the protein tip. Substitution of the Archease metal-binding residues drastically reduced Archease-dependent activation of RtcB. Thus, evolution has sought to co-express archease and rtcB by creating a tRNA splicing operon.
A tRNA splicing operon: Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation.,Desai KK, Cheng CL, Bingman CA, Phillips GN Jr, Raines RT Nucleic Acids Res. 2014 Apr 1;42(6):3931-42. doi: 10.1093/nar/gkt1375. Epub 2014 , Jan 16. PMID:24435797[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Desai KK, Cheng CL, Bingman CA, Phillips GN Jr, Raines RT. A tRNA splicing operon: Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation. Nucleic Acids Res. 2014 Apr 1;42(6):3931-42. doi: 10.1093/nar/gkt1375. Epub 2014 , Jan 16. PMID:24435797 doi:http://dx.doi.org/10.1093/nar/gkt1375
