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2c2r
From Proteopedia
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EFFICIENT AND HIGH FIDELITY INCORPORATION OF DCTP OPPOSITE 7,8-DIHYDRO-8-OXODEOXYGUANOSINE BY SULFOLOBUS SOLFATARICUS DNA POLYMERASE DPO4
Overview
DNA polymerases insert dATP opposite the oxidative damage product, 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) instead of dCTP, to the extent of, >90% with some polymerases. Steady-state kinetics with the Y-family, Sulfolobus solfataricus DNA polymerase IV (Dpo4) showed 90-fold higher, incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher, efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The, catalytic efficiency for these events (with dCTP or C) was similar for G, and 8-oxoG templates. Mass spectral analysis of extended DNA primers, showed >/=95% incorporation of dCTP > dATP opposite 8-oxoG., Pre-steady-state kinetics showed faster rates of dCTP incorporation, opposite 8-oxoG than G. The measured K(d)(,dCTP) was 15-fold lower for an, ... [(full description)]
About this Structure
2C2R is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with CA and DCT as [ligands]. Active as [DNA-directed DNA polymerase], with EC number [2.7.7.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Efficient and high fidelity incorporation of dCTP opposite 7,8-dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA polymerase Dpo4., Zang H, Irimia A, Choi JY, Angel KC, Loukachevitch LV, Egli M, Guengerich FP, J Biol Chem. 2006 Jan 27;281(4):2358-72. Epub 2005 Nov 22. PMID:16306039
Page seeded by OCA on Tue Oct 30 10:48:59 2007
Categories: DNA-directed DNA polymerase | Single protein | Sulfolobus solfataricus | Egli, M. | Irimia, A. | Loukachevitch, L.V. | CA | DCT | 7 | 8-dihydro-8-oxodeoxyguanosine | Calcium | Dna damage | Dna repair | Dna replication | Dna-binding | Dna-directed dna polymerase | Magnesium | Metal-binding | Mutator protein | Nucleotidyltransferase | P2 dna polymerase iv | Transferase | Translesion dna polymerase
