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2c7n

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Revision as of 08:38, 30 October 2007 by OCA (Talk | contribs)
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2c7n, resolution 2.10Å

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HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN

Overview

The interaction between ubiquitinated proteins and intracellular proteins, harboring ubiquitin binding domains (UBDs) is critical to a multitude of, cellular processes. Here, we report that Rabex-5, a guanine nucleotide, exchange factor for Rab5, binds to Ub through two independent UBDs. These, UBDs determine a number of properties of Rabex-5, including its coupled, monoubiquitination and interaction in vivo with ubiquitinated EGFRs., Structural and biochemical characterization of the UBDs of Rabex-5, revealed that one of them (MIU, motif interacting with ubiquitin) binds to, Ub with modes superimposable to those of the UIM (ubiquitin-interacting, motif):Ub interaction, although in the opposite orientation. The other, UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of ... [(full description)]

About this Structure

2C7N is a [Protein complex] structure of sequences from [Homo sapiens] with ZN as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin., Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR, Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. PMID:16499958

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