Function
Methane monooxygenase (MMO) catalyzes the oxidation of the C-H bond of methane and other alkanes. MMO catalyzes the conversion of CH4 and O2 to CH3OH and H2O using NADPH as reducing agent. The soluble MMO (sMMO) has Fe-O-Fe in its active site while the particulate MMO (pMMO) has Cu in it. MMO is composed of 3 subunits. Subunit α is the hydroxylase and contains the di-iron active site. Subunit β is a reductase which contains the co-factors FAD and 2Fe-2S complex. Subunit γ which is involved in coupling. MMO is found in methanotropic bacteria.
Disease
Relevance
Structural highlights
3D structures of methane monooxygenase
Updated on 23-June-2014
1mmo, 1mty, 1fyz, 1fz0, 1fz1, 1fz2, 1fz3, 1fz4, 1fz5, 1fz6, 1fz7, 1fzh, 1fzi, 1xu5 – McMMO α+β+γ subunits + Fe – Methylococcus capsulatus
1xmf – McMMO α+β+γ subunits + Mn
1xmh – McMMO α+β+γ subunits + Co
1xmg – McMMO α+β+γ subunits
1mhy, 1mhz – MtMMO α+β+γ subunits + Fe – Methylosinus trichosporium
sMMO complexes
1fz8 – McMMO α+β+γ subunits + Fe + dibromomethane
1xvc – McMMO α+β+γ subunits + Fe + bromoethane + bromopentane
1xvb – McMMO α+β+γ subunits + Fe + bromoethane + bromopropane + bromobutane + bromohexanol
1fz9 – McMMO α+β+γ subunits + Fe + iodoethane
1xvd – McMMO α+β+γ subunits + Fe + fluorophenol
1xve – McMMO α+β+γ subunits + Fe + bromobutenol
1xvf – McMMO α+β+γ subunits + Fe + chloropropanol
1xvg – McMMO α+β+γ subunits + Fe + bromoethanol
1xu3 – McMMO α+β+γ subunits + Fe + bromophenol
4gam – McMMO α+β+γ subunits + Fe + MMO regulatory protein B
sMMO γ subunit (MMO reductase, MMOR)
1jqr – McMMOR N terminal + Fe2S2 - NMR
[1tvc]] – McMMOR C terminal + FAD derivative - NMR
References
