2chq
From Proteopedia
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REPLICATION FACTOR C ADPNP COMPLEX
Overview
We have investigated the communication between subunits in replication, factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed, arginine finger in the small subunits results in a complex that can still, bind ATP but has impaired clamp-loading activity, a process that normally, only requires binding of nucleotide. The small subunit alone forms a, hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A, conformational change associated with nucleotide binding may relate to the, opening of PCNA rings by RFC during the loading reaction. The structures, also reveal the importance of the N-terminal helix of each subunit at the, ATP-binding site. Analysis of mutant protein complexes containing ... [(full description)]
About this Structure
2CHQ is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with ANP as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Communication between subunits within an archaeal clamp-loader complex., Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB, EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222
Page seeded by OCA on Tue Oct 30 13:25:58 2007
