2clc
From Proteopedia
|
CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP (2)
Overview
We present a new design for a fluorescence microspectrophotometer for use, in kinetic crystallography in combination with x-ray diffraction, experiments. The FLUMIX device (Fluorescence spectroscopy to monitor, intermediates in x-ray crystallography) is built for 0 degrees, fluorescence detection, which has several advantages in comparison to a, conventional fluorometer with 90 degrees design. Due to the reduced, spatial requirements and the need for only one objective, the system is, highly versatile, easy to handle, and can be used for many different, applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly, in a hanging drop crystallization setup. The FLUMIX device can be combined, with most x-ray ... [(full description)]
About this Structure
2CLC is a [Single protein] structure of sequence from [Homo sapiens] with MG, XY2 and GTP as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction., Klink BU, Goody RS, Scheidig AJ, Biophys J. 2006 Aug 1;91(3):981-92. Epub 2006 May 12. PMID:16698776
Page seeded by OCA on Tue Oct 30 12:17:45 2007
Categories: Homo sapiens | Single protein | Goody, R.S. | Klink, B.U. | Scheidig, A.J. | GTP | MG | XY2 | Caged gtp | Disease mutation | Fluorescence | Golgi apparatus | Gtp | Gtp-binding | Guanine nucleotide binding protein | Intermediate | Lipoprotein | Membrane | Methylation | Nucleotide binding protein | Nucleotide-binding | Palmitate | Prenylation | Proto-oncogene | R-caged gtp
