2j8c

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2j8c, resolution 1.87Å

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X-RAY HIGH RESOLUTION STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTER FROM RB. SPHAEROIDES AT PH 8 IN THE NEUTRAL STATE

Overview

The structure of the photosynthetic reaction-center from Rhodobacter, sphaeroides has been determined at four different pH values (6.5, 8.0, 9.0, 10.0) in the neutral and in charge separated states. At pH 8.0, in, the neutral state, we obtain a resolution of 1.87 A, which is the best, ever reported for the bacterial reaction center protein. Our, crystallographic data confirm the existence of two different binding, positions of the secondary quinone (Q(B)). We observe a new orientation of, Q(B) in its distal position, which shows no ring-flip compared to the, orientation in the proximal position. Datasets collected for the different, pH values show a pH-dependence of the population of the proximal position., The new orientation of Q(B) in the distal position and the pH-dependence, could be ... [(full description)]

About this Structure

2J8C is a [Protein complex] structure of sequences from [Rhodobacter sphaeroides] with PO4, FE, BCL, LDA, BPH, U10, HTO, SPO, CDL, PC1, GGD and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

pH Modulates the Quinone Position in the Photosynthetic Reaction Center from Rhodobacter sphaeroides in the Neutral and Charge Separated States., Koepke J, Krammer EM, Klingen AR, Sebban P, Ullmann GM, Fritzsch G, J Mol Biol. 2007 Aug 10;371(2):396-409. Epub 2007 May 10. PMID:17570397

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