2pot

From Proteopedia

proteopedia linkproteopedia link

Template:STRUCTURE 2pot

Contents 1 tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine 2 Function 3 About this Structure 4 Reference

tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine

Template:ABSTRACT PUBMED 17949745

Function

[TGT_ZYMMO] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]

About this Structure

2pot is a 1 chain structure with sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.

Reference

• Tidten N, Stengl B, Heine A, Garcia GA, Klebe G, Reuter K. Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies. J Mol Biol. 2007 Nov 30;374(3):764-76. Epub 2007 Oct 22. PMID:17949745 doi:10.1016/j.jmb.2007.09.062