Endonuclease

From Proteopedia

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Template:STRUCTURE 1rva Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN. See also

• Apurinic-Apyrimidinic Endonuclease-1
  
• DNA repair protein human endonuclease VIII-like 1 (NEIL1) .

3D structures of endonuclease

Updated on 24-November-2014 {{#tree:id=OrganizedByTopic|openlevels=0|

• ENN-I

• • 1bix - hENN – human
    
  • 1cfr – ENN – Citrobacter freundii
    
  • 1af5 - CrENN – Chlamydomonas reinhardtii
    
  • 1fzr, 1m0d, 1m0i, 3cae – T7ENN - Enterobacteria phage T7
    
  • 2g7e, 2g7f – VcENN – Vibrio cholera
    
  • 2vnd - VcENN (mutant)
    
  • 2pu3 - VsENN – Vibrio salmonicida

• ENN-II

• • 1pvi, 1pvu - ENN – Proteus vulgaris
    
  • 2wsh - T4ENN (mutant) - Enterobacteria phage T4
    
  • 3w52 – ENN – Arabidopsis thaliana
    

• ENN-III

• • 2abk, 1ako - EcENN – Escherichia coli
    
  • 1orn, 1orp, 1p59 - GsENN + DNA – Geobacillus stearothermophilus

• ENN-IV

• • 1qtw - EcENN
    
  • 2nqh - EcENN (mutant)
    
  • 1qum – EcENN + DNA
    
  • 2nqj, 2nq9, 4k1g - EcENN (mutant) + DNA
    
  • 1xp3 - ENN – Bacillus anthracis
    
  • 3aal - GkENN – Geobacillus kaustophilus
    
  • 3aam - TtENN – Thermus thermophilus

• ENN-V

• • 1eni, 1enj, 1enk, 2end - T4ENN
    
  • 1vas - T4ENN + DNA
    
  • 2w35, 2w36, 4b20 - TmENN + DNA – Thermotoga maritima
    
  • 3hd0 - TmENN
    
  • 3ga2 - BsENN (mutant) – Bacillus subtilis
    
  • 3goc - ENN – Streptomyces avermitilis
    
  • 2fcc – T4ENN + DNA
    

• ENN-VII

• • 1en7, 1e7d - T4ENN
    
  • 1e7l - T4ENN (mutant)
    
  • 2qnc, 2qnf - T4ENN (mutant) + DNA

• ENN-VIII

• • 1q39, 1q39 - EcENN
    
  • 1q3b, 1q3c - EcENN (mutant)
    
  • 1tdh – hENN
    
  • 1bix – hENN + DNA
    
  • 1k3x, 2ea0, 1k3w, 1k3x, 2ea0, 2opf, 2oq4 - EcENN + DNA
    
  • 2opf, 2oq4 - EcENN (mutant) + DNA

• EcoKI ENN

• • 2ar0 – EcENN (mutant)
    

• Ecoo109I ENN

• • 1wtd - EcENN
    
  • 1wte – EcENN + DNA
    

• EcoRI ENN

• • 1qc9 - EcENN
    
  • 1eri, 1ckq, 1cl8, 1qps – EcENN + DNA
    
  • 1qrh, 1qri, 2oxv - EcENN (mutant) + DNA

• EcoRII ENN

• • 1na6 – EcENN
    
  • 3hqf, 3hqg - EnENN + DNA

• EcoRV ENN

• • 1rve, 1az3 – EcENN
    
  • 1az4 - EcENN (mutant)
    
  • 2rve, 4rve, 1rva, 1rvb, 1rvc, 1az0, 1bsu, 1bua, 1bgb, 1rv5, 1b94, 1b95, 1eo3, 1eo4, 1eon, 1eoo, 1eop, 2b0d, 2b0e - EcENN + DNA
    
  • 1bss, 1b96, 1b97, 1stx, 1suz, 1sx5, 1sx8, 2ge5 - EcENN (mutant) + DNA

• BamHI ENN

• • 1bam – BaENN – Bacillus amyloliquefaciens
    
  • 1bhm, 3bam, 2bam, 1esg - BaENN + DNA

• FokI ENN

• • 1fok – PoENN + DNA – Planomicrobium okeanokoites
    
  • 2fok – PoENN

• PvuII ENN

• • 3pvi - PvENN (mutant) + DNA – Proteus vulgaris
    
  • 1k0z, 1h56, 3ksk - PvENN
    
  • 1ni0 - PvENN (mutant)
    
  • 2pvi, 1eyu, 1f0o - PvENN + DNA

• tRNA-splicing ENN

• • 1a79, 1a76, 1a77 - MjENN – Methanocaldococcus jannaschii
    
  • 2cv8 - ENN – Sulfolobus tokodaii
    
  • 2gw6 – hENN subunit sen15 – NMR
    
  • 2gjw - AfENN + RNA – Archaeoglobus fulgidus
    
  • 1r0v, 1r11 – AfENN
  • 2ohc, 2ohe - TaENN – Thermoplasma acidophilum
    
  • 2zyz – ENN – Pyrobaculum aerophilum
    
  • 3iey - ENN + NEQ261 – Nanoarchaeum equitans
    
  • 3ajv - ApENN (mutant) – Aeropyrum pernix
    
  • 3p1y - ApENN /AfENN
    
  • 3p1z - ApENN + protein

• VSR ENN

• • 1vsr – EcENN
    
  • 1cw0 - EcENN + DNA

• BglI ENN

• • 1dmu, 1d2i – BsENN + DNA
    
  • 1dfm - BsENN (mutant) + DNA

• BglII ENN

• • 1es8 - BsENN

• MunI ENN

• • 1d02 - ENN (mutant) + DNA - Mycoplasma

• Sm ENN

• • 1smn, 1qae, 1ql0, 1g8t – ENN – Serratia marcescens

• NaeI ENN

• • 1ev7 - LaENN – Lechevalieria aerocolonigenes
    
  • 1iaw - LaENN + DNA

• NgoMIV ENN

• • 1fiu, 4abt - ENN + DNA – Neisseria gonorrheae

• NEI ENN

• • 1tdh - hENN

• BsoBI ENN

• • 1dc1 - GsENN + DNA

• Bse634I ENN

• • 1knv – GsENN
    
  • 3v1z – GsENN + DNA
    
  • 3v20, 3v21 - GsENN (mutant) + DNA

• HincII ENN

• • 1kc6, 1tw8, 1xhu, 1xhv, 1tx3, 2gie - HiENN + DNA – Haemophilus influenza
    
  • 2gig, 2gih, 2gii, 2gij, 3e3y, 3e40, 3e41, 3e42, 3e43, 3e44, 3e45, 3ebc - HiENN (mutant) + DNA
    
  • [2aud]] – HiENN

• HinDIII ENN

• • 2e52, 3a4k - HiENN + DNA

• HinP1I ENN

• • 1ynm – HiENN
    
  • 2fkc, 2fkh, 2fl3, 2flc - HiENN + DNA

• Flap ENN

• • 1mc8 - ENN (mutant) – Pyrococcus horikoshii
    
  • 3ory– ENN – Desulfurococcus amylolyticus
    
  • 3q8k, 3q8l, 3q8m - hENN + DNA
    
  • 1rxv, 1rxw - AfENN + DNA
    
  • 1ul1 - hENN + PCNA
    
  • 2izo - SsENN + PCNA – Sulfolobus solfataricus
    
  • 1a76, 1a77 - MjENN
    
  • 1b43 – PfENN – Pyrococcus furiosus
    
  • 1rxz - AfENN + PCNA
    

• DNA mismatch ENN

• • 1odg - EcENN + DNA
    
  • 1h7u – hENN
    

• Vvn ENN

• • 1ouo – VvENN – Vibrio vulnificus
    
  • 1oup, 2ivk - VvENN + DNA

• BfiI ENN

• • 2c1l – ENN – Bacillus firmus

• SfiI ENN

• • 2ezv, 2f03 – ENN + DNA – synthetic

• Ecl18kI ENN

• • 2fqz, 2gb7 – ENN + DNA – Enterobacter cloacae

• SdaI ENN

• • 2ixs – ENN – Streptomyces diastaticus

• PabI ENN

• • 2dvy – ENN – Pyrococcus abyssi

• PspGI ENN

• • 3bm3 – ENN – Pyrococcus

• NHN ENN

• • 2qgp – ENN – Geobacter metallireducens

• UV damage ENN

• • 2j6v, 3bzg, 3c0s – TtENN
    
  • 3bzj, 3c0l, 3c0q - TtENN (mutant)
    
  • 4gle - SaENN + DNA
    

• NotI ENN

• • 3bvq - NoENN – Nocardia otitidiscaviarum
    
  • 3c25 - NoENN + DNA

• BpujI ENN

• • 2vla, 3s1s – ENN – Bacillus pumilus

• Sau3AI ENN

• • 2reu – ENN C terminal – Staphylococcus aureus

• HpyI ENN

• • 3fc3, [[3gox – HpENN99 + DNA – Helicobacter pylori
    
  • 3or3 – HpENN88 + DNA

• PacI ENN

• • 3ldy, 3m7k - PaENN + DNA – Psdeudomonas alcaligenes

• Crossover junction ENN

• • 2kpf - ENN – mouse

• OkrAI ENN

• • 3odh - ENN + DNA – Oceanobacter kriegii

• BspD6I ENN

• • 2ewf - ENN – Bacillus

• ThaI ENN

• • 3ndh - TaENN + DNA

• Xpf ENN

• • 1z00, 2a1j - hENN C terminal + DNA excision repair protein
    
  • 2bgw - ApENN + DNA
    
  • 2bhn - ApENN
    

• Intron-encoded endonuclease

• I-PpoI ENN

• • 1a74, 1ipp, 1a73, 1cz0 - PpENN + DNA – Physarum polycephalum
    
  • 1cyq, 1evw, 2o6m - PpENN (mutant) + DNA
    
  • 1evx - PpENN

• I-CreI ENN

• • 1bp7, 1g9y, 1g9z, 1n3e, 1n3f, 2vbj, 2vbl, 2vbn, 2vbo, 4aqu, 4aqx - CrENN + DNA
    
  • 1u0c, 1u0d, 1t9i, 1t9j, 2i3p, 2i3q, 4aab, 4aad, 4aae, 4aaf, 4aag - CrENN (mutant) + DNA
    
  • 2o7m - CrENN

• I-CeuI ENN

• • 2ex5 - ENN (mutant) + DNA – Chlamydomonas eugametos

• I-DmoI ENN

• • 1b24, 2vs7, 2vs8 - DmENN + DNA – Desulfurococcus mobilis
    
  • 1mow – DmENN/CrENN + DNA

• I-AniI ENN

• • 1p8k, 2qoj, 3uvf - EnENN + DNA – Emericella nidulans
    
  • 3eh8 - EnENN (mutant) + DNA

• I-HmuI ENN

• • 1u3e - ENN + DNA – Bacillus phage spo1

• I-TevI ENN

• • 1i3j, 1t2t - T4ENN + DNA
    
  • 1ln0, 1mk0 – T4ENN (mutant)

• I-SceI ENN

• • 1dfa, 1ef0 – yENN – yeast
    
  • 1gpp – yENN (mutant)
    
  • 1lws, 1lwt, 1r7m, 3c0w, 3c0x, 3ool - yENN + DNA
    
  • 3oor - yENN (mutant) + DNA

• I-PkoII ENN

• • 2cw7, 2cw8 – ENN – Thermococcus kodakarensis

• I-MspI ENN

• • 1sa3, 1yfi, 2fld – ENN + DNA – Moraxella

• I-PfuI ENN

• • 1dq3 – PfENN

• I-Tsp061I ENN

• • 2dch – ENN – Thermoproteus

• I-Vdi141I ENN

• • 3e54 – ENN – Vulcanisaeta distribute

• I-MtuI ENN

• • 3ifj, 3igd - MtENN (mutant) – Mycobacterium tuberculosis
    
  • 2l8l - MtENN

• I-MsoI ENN

• • 1m5x, 3fd2 – MoENN + DNA – Monomastix
    
  • 3ko2 – MoENN (mutant) + DNA

• I-GzeMII ENN

• • 4efj – ENN + DNA – Gibberella zeae

• I-tRNA ENN

• • 4fz2 – MoENN – Candidatus micrarchaeum
    

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