4lip
From Proteopedia
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PSEUDOMONAS LIPASE COMPLEXED WITH RC-(RP, SP)-DIBUTYLCARBAMOYLGLYCERO-3-O-BUTYLPHOSPHONATE
Overview
To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and, Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates, of different alkyl chain lengths. P. cepacia lipase was most rapidly, inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl, octylphosphonate (Rc-trioctyl) with an inactivation half-time of 75 min, while that for the, Sc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octyl-phosphonate, (Sc-trioctyl) compound was 530 min. X-ray structures were obtained of P., cepacia lipase after reaction with Rc-trioctyl to 0.29-nm resolution at pH, 4 and covalently modified with, Rc-(Rp,Sp)-1,2-dibutylcarbamoylglycero-3-O-p-nitrophenyl butyl-phosphonate, (Rc-tributyl) to 0.175-nm resolution ... [(full description)]
About this Structure
4LIP is a [Single protein] structure of sequence from [Burkholderia cepacia] with CA and CCP as [ligands]. Active as [Triacylglycerol lipase], with EC number [3.1.1.3]. Structure known Active Sites: ACD, ACE, OXD and OXE. Full crystallographic information is available from [OCA].
Reference
Structural basis of the chiral selectivity of Pseudomonas cepacia lipase., Lang DA, Mannesse ML, de Haas GH, Verheij HM, Dijkstra BW, Eur J Biochem. 1998 Jun 1;254(2):333-40. PMID:9660188
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