Transposase

Function

Transposases bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. Mu transposase (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA.

Disease

Relevance

Structural highlights

3D structures of transposase

Updated on 07-August-2014

Mu transposase

1tns, 1tnt – MuMuA DNA-binding domain – enterobacteria phage Mu - NMR
1bcm, 1bco – MuMuA core domain
2ezh, 2ezi – MuMuA Iγ subdomain - NMR
2ezl – MuMuA Iβ subdomain - NMR
4fcy – MuMuA + DNA

Hermes transposase

2bw3 – fTra (mutant) - fly
4d1q – fTra (mutant) + DNA

Dra2 transposase

2xm3 – Dra2 + Dra2 transposase binding element + DNA – Deinococcus radiodurans
2xma, 2xqc – Dra2 + DNA
2xo6 – Dra2 (mutant) + DNA

Tn5 transposase

1muh, 1mus, 1mm8, 3ecp, 4dm0 – EcTn5 (mutant) + DNA – Escherichia coli

Mos1 transposase

2f7t – DmMos1 catalytic domain (mutant) – Drosophila mauritiana
3hos, 3hot – DmMos1 (mutant) + DNA
4mda, 4mdb – DmMos1 catalytic domain (mutant) + drug

Tc3 transposase

1tc3, 1u78 – Tc3 DNA-binding domain (mutant) + DNA – Caenorhabditis elegans

References