1h71
From Proteopedia
|
PSYCHROPHILIC PROTEASE FROM PSEUDOALTEROMONAS 'TAC II 18'
Overview
Enzymes from psychrophilic organisms differ from their mesophilic, counterparts in having a lower thermostability and a higher specific, activity at low and moderate temperatures. It is in general accepted that, psychrophilic enzymes are more flexible to allow easy accommodation and, transformation of the substrates at low energy costs. Here, we report the, structures of two crystal forms of the alkaline protease from an Antarctic, Pseudomonas species (PAP), solved to 2.1- and 1.96-A resolution, respectively. Comparative studies of PAP structures with mesophilic, counterparts show that the overall structures are similar but that the, conformation of the substrate-free active site in PAP resembles that of, the substrate-bound region of the mesophilic homolog, with both an, active-site ... [(full description)]
About this Structure
1H71 is a [Single protein] structure of sequence from [Bacteria] with CA and ZN as [ligands]. Active as [Hydrolase], with EC number [3.4.24.40.]. Structure known Active Site: ZNB. Full crystallographic information is available from [OCA].
Reference
Crystal structures of a psychrophilic metalloprotease reveal new insights into catalysis by cold-adapted proteases., Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser R, Van Beeumen J, Proteins. 2003 Mar 1;50(4):636-47. PMID:12577270
Page seeded by OCA on Tue Oct 30 15:30:51 2007
