3a5z

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Template:STRUCTURE 3a5z

Contents 1 Crystal structure of Escherichia coli GenX in complex with elongation factor P 2 Function 3 About this Structure 4 See Also 5 Reference

Crystal structure of Escherichia coli GenX in complex with elongation factor P

Template:ABSTRACT PUBMED 20729861

Function

[C3SGA2_ECOLX] With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF-P 'Lys-34' (By similarity).[HAMAP-Rule:MF_00174] [C3SGD7_ECOLX] Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Modification of Lys-34 is required for alleviation (By similarity).[HAMAP-Rule:MF_00141]

About this Structure

3a5z is a 8 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

See Also

• Elongation factor

Reference

• Yanagisawa T, Sumida T, Ishii R, Takemoto C, Yokoyama S. A paralog of lysyl-tRNA synthetase aminoacylates a conserved lysine residue in translation elongation factor P. Nat Struct Mol Biol. 2010 Sep;17(9):1136-43. Epub 2010 Aug 22. PMID:20729861 doi:10.1038/nsmb.1889