3lf0

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Template:STRUCTURE 3lf0

Contents 1 Crystal structure of the ATP bound Mycobacterium tuberculosis nitrogen regulatory PII protein 2 Function 3 About this Structure 4 Reference

Crystal structure of the ATP bound Mycobacterium tuberculosis nitrogen regulatory PII protein

Template:ABSTRACT PUBMED 20521335

Function

[GLNB_MYCTU] In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed (By similarity).

About this Structure

3lf0 is a 3 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

• Shetty ND, Reddy MC, Palaninathan SK, Owen JL, Sacchettini JC. Crystal structures of the apo and ATP bound Mycobacterium tuberculosis nitrogen regulatory PII protein. Protein Sci. 2010 Aug;19(8):1513-24. PMID:20521335 doi:10.1002/pro.430