3pwe

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Template:STRUCTURE 3pwe

Contents 1 Crystal structure of the E. coli beta clamp mutant R103C, I305C, C260S, C333S at 2.2A resolution 2 Function 3 About this Structure 4 Reference

Crystal structure of the E. coli beta clamp mutant R103C, I305C, C260S, C333S at 2.2A resolution

Template:ABSTRACT PUBMED 21971175

Function

[DPO3B_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.

About this Structure

3pwe is a 2 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA.

Reference

• Paschall CO, Thompson JA, Marzahn MR, Chiraniya A, Hayner JN, O'Donnell M, Robbins AH, McKenna R, Bloom LB. The E. coli clamp loader can actively pry open the beta-sliding clamp. J Biol Chem. 2011 Oct 4. PMID:21971175 doi:10.1074/jbc.M111.268169