3v97

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Template:STRUCTURE 3v97

Contents 1 Crystal structure of bifunctional methyltransferase YcbY (RlmLK) from Escherichia coli, SAH binding 2 Function 3 About this Structure 4 Reference

Crystal structure of bifunctional methyltransferase YcbY (RlmLK) from Escherichia coli, SAH binding

Template:ABSTRACT PUBMED 22362734

Function

[RLMKL_ECOLI] Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits.^{[1] [2] [3]}

About this Structure

3v97 is a 2 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA.

Reference

• Wang KT, Desmolaize B, Nan J, Zhang XW, Li LF, Douthwaite S, Su XD. Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA. Nucleic Acids Res. 2012 Jun;40(11):5138-48. doi: 10.1093/nar/gks160. Epub 2012, Feb 22. PMID:22362734 doi:10.1093/nar/gks160

1. ↑ Lesnyak DV, Sergiev PV, Bogdanov AA, Dontsova OA. Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA. J Mol Biol. 2006 Nov 17;364(1):20-5. Epub 2006 Sep 8. PMID:17010378 doi:S0022-2836(06)01182-X
2. ↑ Kimura S, Ikeuchi Y, Kitahara K, Sakaguchi Y, Suzuki T, Suzuki T. Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity. Nucleic Acids Res. 2012 May;40(9):4071-85. doi: 10.1093/nar/gkr1287. Epub 2011, Dec 30. PMID:22210896 doi:10.1093/nar/gkr1287
3. ↑ Wang KT, Desmolaize B, Nan J, Zhang XW, Li LF, Douthwaite S, Su XD. Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA. Nucleic Acids Res. 2012 Jun;40(11):5138-48. doi: 10.1093/nar/gks160. Epub 2012, Feb 22. PMID:22362734 doi:10.1093/nar/gks160