4iqp
From Proteopedia
Contents |
Crystal Structure of HCRA-W1266A
Template:ABSTRACT PUBMED 23670557
Function
[BXA1_CLOBO] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
About this Structure
4iqp is a 1 chain structure with sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
Reference
- Przedpelski A, Tepp WH, Kroken AR, Fu Z, Kim JJ, Johnson EA, Barbieri JT. Enhancing the Protective Immune Response against Botulism. Infect Immun. 2013 May 13. PMID:23670557 doi:10.1128/IAI.00382-13
