3wnm

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Template:STRUCTURE 3wnm

Contents

D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoheptaose

Template:ABSTRACT PUBMED 23908050

Function

[CTA1_BACCI] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.[1]

About this Structure

3wnm is a 1 chain structure. Full crystallographic information is available from OCA.

Reference

  • Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K. Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050 doi:http://dx.doi.org/10.1107/S174430911301991X
  1. ↑ Oguma T, Tobe K, Kobayashi M. Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides. FEBS Lett. 1994 May 30;345(2-3):135-8. PMID:7515357

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