1cfm is a 3 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A truncated form of cytochrome f from Chlamydomonas reinhardtii (an important eukaryotic model organism for photosynthetic electron transfer studies) has been crystallized (space group P2(1)2(1)2(1); three molecules/asymmetric unit) and its structure determined to 2.0 A resolution by molecular replacement using the coordinates of a truncated turnip cytochrome f as a model. The structure displays the same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation by the alpha-amino group of tyrosine 1, (b) a cluster of lysine residues (proposed docking site of plastocyanin), and (c) the presence of a chain of seven water molecules bound to conserved residues and extending between the heme pocket and K58 and K66 at the lysine cluster. For this array of waters, we propose a structural role. Two cytochrome f molecules are related by a noncrystallographic symmetry operator which is a distorted proper 2-fold rotation. This may represent the dimeric relation of the monomers in situ; however, the heme orientation suggested by this model is not consistent with previous EPR measurements on oriented membranes.
X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii.,Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:10869174[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA. X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii. Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:10869174
