1b8j is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the vanadate ion are stabilized by interactions with both Arg-166 and the zinc metal ions of the active site. This structural complex supports the in-line displacement mechanism of phosphomonoester hydrolysis by alkaline phosphatase and provides a model for the proposed transition state in the enzyme-catalyzed reaction.
A model of the transition state in the alkaline phosphatase reaction.,Holtz KM, Stec B, Kantrowitz ER J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Holtz KM, Stec B, Kantrowitz ER. A model of the transition state in the alkaline phosphatase reaction. J Biol Chem. 1999 Mar 26;274(13):8351-4. PMID:10085061
