This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2j6a
From Proteopedia
|
CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.
Overview
Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with, eRF3 and GTP, is methylated on a functionally crucial Gln residue by the, S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show, that eRF1 methylation, in addition to these previously characterized, components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression, in Escherichia coli of Ynr046w and Ydr140w allows the latter to be, recovered in soluble form rather than as inclusion bodies, and the two, proteins co-purify on nickel-nitrilotriacetic acid chromatography when, Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been, determined to 1.7 A resolution. It comprises a zinc-binding domain built, from both the N- and C-terminal sequences and an inserted domain, ... [(full description)]
About this Structure
2J6A is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with ZN and EDO as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308
Page seeded by OCA on Tue Oct 30 17:25:19 2007
