1u8x
From Proteopedia
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CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE
Overview
GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.
About this Structure
1U8X is a Single protein structure of sequence from Bacillus subtilis with , and as ligands. This structure supersedes the now removed PDB entry 1NRH. Active as Maltose-6'-phosphate glucosidase, with EC number 3.2.1.122 Full crystallographic information is available from OCA.
Reference
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:15341727
Page seeded by OCA on Thu Feb 21 15:21:51 2008
Categories: Bacillus subtilis | Maltose-6'-phosphate glucosidase | Single protein | Anderson, W F. | Collart, F. | MCSG, Midwest Center for Structural Genomics. | Rajan, S S. | Yang, X. | G6P | MN | NAD | Glucosidase | Mcsg | Midwest center for structural genomics | Nad-dependent | Protein structure initiative | Psi | Structural genomics