1wnu
From Proteopedia
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Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine
Overview
AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).
About this Structure
1WNU is a Single protein structure of sequence from Pyrococcus horikoshii with and as ligands. Full crystallographic information is available from OCA.
Reference
Molecular basis of alanine discrimination in editing site., Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I, Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11669-74. Epub 2005 Aug 8. PMID:16087889
Page seeded by OCA on Thu Feb 21 15:46:21 2008
Categories: Pyrococcus horikoshii | Single protein | Nakashima, T. | Okada, A. | Sokabe, M. | Tanaka, I. | Yao, M. | SER | ZN | Hydrolase
