4j0l

From Proteopedia

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Template:STRUCTURE 4j0l

Contents 1 Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules 2 Function 3 About this Structure 4 Reference

Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules

Template:ABSTRACT PUBMED 23871710

Function

[CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.^{[1] [2] [3]}

About this Structure

4j0l is a 1 chain structure with sequence from Secale cereale. Full crystallographic information is available from OCA.

Reference

• Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710 doi:http://dx.doi.org/10.1016/j.febslet.2013.07.008

1. ↑ Ohnuma T, Yagi M, Yamagami T, Taira T, Aso Y, Ishiguro M. Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c. Biosci Biotechnol Biochem. 2002 Feb;66(2):277-84. PMID:11999399
2. ↑ Taira T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed. Biosci Biotechnol Biochem. 2001 Dec;65(12):2710-8. PMID:11826968
3. ↑ Taira T, Ohnuma T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci Biotechnol Biochem. 2002 May;66(5):970-7. PMID:12092848