1zro

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spinqualitylabelsall models 1zro, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of EBA-175 Region II (RII) crystallized in the presence of (alpha)2,3-sialyllactose

Overview

Erythrocyte binding antigen 175 (EBA-175) is a P. falciparum protein that binds the major glycoprotein found on human erythrocytes, glycophorin A, during invasion. Here we present the crystal structure of the erythrocyte binding domain of EBA-175, RII, which has been established as a vaccine candidate. Binding sites for the heavily sialylated receptor glycophorin A are proposed based on a complex of RII with a glycan that contains the essential components required for binding. The dimeric organization of RII displays two prominent channels that contain four of the six observed glycan binding sites. Each monomer consists of two Duffy binding-like (DBL) domains (F1 and F2). F2 more prominently lines the channels and makes the majority of the glycan contacts, underscoring its role in cytoadherence and in antigenic variation in malaria. Our studies provide insight into the mechanism of erythrocyte invasion by the malaria parasite and aid in rational drug design and vaccines.

About this Structure

1ZRO is a Single protein structure of sequence from Plasmodium falciparum with and as ligands. Full crystallographic information is available from OCA.

Reference

Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum., Tolia NH, Enemark EJ, Sim BK, Joshua-Tor L, Cell. 2005 Jul 29;122(2):183-93. PMID:16051144

Page seeded by OCA on Thu Feb 21 16:18:25 2008