3puh

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Template:STRUCTURE 3puh

Contents 1 Cocaine Esterase, wild-type biologically active dimer 2 Function 3 About this Structure 4 Reference

Cocaine Esterase, wild-type biologically active dimer

Template:ABSTRACT PUBMED 21890748

Function

[COCE_RHOSM] Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants. Also efficiently hydrolyzes cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. Is able to prevent cocaine-induced convulsions and lethality in rat.^{[1] [2] [3]}

About this Structure

3puh is a 2 chain structure with sequence from Rhodococcus sp. mb1 'bresler 1999'. Full crystallographic information is available from OCA.

Reference

• Narasimhan D, Collins GT, Nance MR, Nichols J, Edwald E, Chan J, Ko MC, Woods JH, Tesmer JJ, Sunahara RK. Subunit Stabilization and PEGylation of Cocaine Esterase Improves In Vivo Residence Time. Mol Pharmacol. 2011 Sep 2. PMID:21890748 doi:10.1124/mol.111.074997

1. ↑ Bresler MM, Rosser SJ, Basran A, Bruce NC. Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1. Appl Environ Microbiol. 2000 Mar;66(3):904-8. PMID:10698749
2. ↑ Cooper ZD, Narasimhan D, Sunahara RK, Mierzejewski P, Jutkiewicz EM, Larsen NA, Wilson IA, Landry DW, Woods JH. Rapid and robust protection against cocaine-induced lethality in rats by the bacterial cocaine esterase. Mol Pharmacol. 2006 Dec;70(6):1885-91. Epub 2006 Sep 12. PMID:16968810 doi:10.1124/mol.106.025999
3. ↑ Turner JM, Larsen NA, Basran A, Barbas CF 3rd, Bruce NC, Wilson IA, Lerner RA. Biochemical characterization and structural analysis of a highly proficient cocaine esterase. Biochemistry. 2002 Oct 15;41(41):12297-307. PMID:12369817