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4ft8
From Proteopedia
Revision as of 07:16, 24 September 2014 by OCA (Talk | contribs)
4ft8 is a 2 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
The crystal structure of cyclic AMP-catabolite activator protein (CAP) from Escherichia coli containing cobalt(II) chloride and ammonium sulfate is reported at 1.97 A resolution. Each of the two CAP subunits in the asymmetric unit binds one cobalt(II) ion, in each case coordinated by N-terminal domain residues His19, His21 and Glu96 plus an additional acidic residue contributed via a crystal contact. The three identified N-terminal domain cobalt-binding residues are part of a region of CAP that is important for transcription activation at class II CAP-dependent promoters. Sulfate anions mediate additional crystal lattice contacts and occupy sites corresponding to DNA backbone phosphate positions in CAP-DNA complex structures.
Structure of catabolite activator protein with cobalt(II) and sulfate.,Rao RR, Lawson CL Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):560-3. doi:, 10.1107/S2053230X14005366. Epub 2014 Apr 15. PMID:24817710[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
