4noj

From Proteopedia

proteopedia linkproteopedia link

Template:STRUCTURE 4noj

Contents 1 Crystal structure of the mature form of asparaginyl endopeptidase (AEP)/Legumain activated at pH 3.5 2 Function 3 About this Structure 4 Reference

Crystal structure of the mature form of asparaginyl endopeptidase (AEP)/Legumain activated at pH 3.5

Template:ABSTRACT PUBMED 24407422

Function

[LGMN_MOUSE] Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.^{[1] [2] [3]}

About this Structure

4noj is a 1 chain structure. Full crystallographic information is available from OCA.

Reference

• Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung LW, Huang W, Liu L, Ye K, Ouyang S, Cheng G, Liu ZJ. Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage. Cell Res. 2014 Jan 10. doi: 10.1038/cr.2014.4. PMID:24407422 doi:http://dx.doi.org/10.1038/cr.2014.4

1. ↑ Chen JM, Dando PM, Stevens RA, Fortunato M, Barrett AJ. Cloning and expression of mouse legumain, a lysosomal endopeptidase. Biochem J. 1998 Oct 1;335 ( Pt 1):111-7. PMID:9742219
2. ↑ Morita Y, Araki H, Sugimoto T, Takeuchi K, Yamane T, Maeda T, Yamamoto Y, Nishi K, Asano M, Shirahama-Noda K, Nishimura M, Uzu T, Hara-Nishimura I, Koya D, Kashiwagi A, Ohkubo I. Legumain/asparaginyl endopeptidase controls extracellular matrix remodeling through the degradation of fibronectin in mouse renal proximal tubular cells. FEBS Lett. 2007 Apr 3;581(7):1417-24. Epub 2007 Mar 5. PMID:17350006 doi:http://dx.doi.org/10.1016/j.febslet.2007.02.064
3. ↑ Miller G, Matthews SP, Reinheckel T, Fleming S, Watts C. Asparagine endopeptidase is required for normal kidney physiology and homeostasis. FASEB J. 2011 May;25(5):1606-17. doi: 10.1096/fj.10-172312. Epub 2011 Feb 3. PMID:21292981 doi:http://dx.doi.org/10.1096/fj.10-172312