3rtr is a 8 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
How RING E3 ligases mediate E2-to-substrate ubiquitin-like protein (UBL) transfer remains unknown. Here we address how the RING E3 RBX1 positions NEDD8's E2 (UBC12) and substrate (CUL1). We find that existing structures are incompatible with CUL1 NEDD8ylation and report a new conformation of RBX1 that places UBC12 adjacent to CUL1. We propose RING domain rotation as a general mechanism for UBL transfer for the largest family of E3s.
A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases.,Calabrese MF, Scott DC, Duda DM, Grace CR, Kurinov I, Kriwacki RW, Schulman BA Nat Struct Mol Biol. 2011 Jul 17;18(8):947-9. doi: 10.1038/nsmb.2086. PMID:21765416[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
