1wu9 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
EB1 proteins bind to microtubule ends where they act in concert with other components, including the adenomatous polyposis coli (APC) tumor suppressor, to regulate the microtubule filament system. We find that EB1 is a stable dimer with a parallel coiled coil and show that dimerization is essential for the formation of its C-terminal domain (EB1-C). The crystal structure of EB1-C reveals a highly conserved surface patch with a deep hydrophobic cavity at its center. EB1-C binds two copies of an APC-derived C-terminal peptide (C-APCp1) with equal 5 microM affinity. The conserved APC Ile2805-Pro2806 sequence motif serves as an anchor for the interaction of C-APCp1 with the hydrophobic cavity of EB1-C. Phosphorylation of the conserved Cdc2 site Ser2789-Lys2792 in C-APCp1 reduces binding four-fold, indicating that the interaction APC-EB1 is post-translationally regulated in cells. Our findings provide a basis for understanding the dynamic crosstalk of EB1 proteins with their molecular targets in eukaryotic organisms.
Structural insights into the EB1-APC interaction.,Honnappa S, John CM, Kostrewa D, Winkler FK, Steinmetz MO EMBO J. 2005 Jan 26;24(2):261-9. Epub 2004 Dec 23. PMID:15616574[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Honnappa S, John CM, Kostrewa D, Winkler FK, Steinmetz MO. Structural insights into the EB1-APC interaction. EMBO J. 2005 Jan 26;24(2):261-9. Epub 2004 Dec 23. PMID:15616574
