Publication Abstract from PubMed
Tensin is a cytoskeletal protein that links integrins to the actin cytoskeleton at sites of cell-matrix adhesion. Here we describe the crystal structure of the phosphotyrosine-binding (PTB) domain of tensin1, and show that it binds integrins in an NPxY-dependent fashion. Alanine mutagenesis of both the PTB domain and integrin tails supports a model of integrin binding similar to that of the PTB-like domain of talin. However, we also show that phosphorylation of the NPxY tyrosine, which disrupts talin binding, has a negligible effect on tensin binding. This suggests that tyrosine phosphorylation of integrin, which occurs during the maturation of focal adhesions, could act as a switch to promote the migration of tensin-integrin complexes into fibronectin-mediated fibrillar adhesions.
Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions.,McCleverty CJ, Lin DC, Liddington RC Protein Sci. 2007 Jun;16(6):1223-9. Epub 2007 May 1. PMID:17473008[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
