3aji

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Template:STRUCTURE 3aji

Contents 1 Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion 2 Function 3 About this Structure 4 See Also 5 Reference

Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion

Template:ABSTRACT PUBMED 21128684

Function

[PSD10_MOUSE] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export (By similarity). [PRS6B_MOUSE] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

About this Structure

3aji is a 4 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA.

See Also

• Ankyrin
• Proteasome

Reference

• Sato S, Mimasu S, Sato A, Hino N, Sakamoto K, Umehara T, Yokoyama S. Crystallographic Study of a Site-Specifically Cross-Linked Protein Complex with a Genetically Incorporated Photoreactive Amino Acid . Biochemistry. 2010 Dec 16. PMID:21128684 doi:10.1021/bi1016183