Publication Abstract from PubMed
Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer.
Cryo-EM model of the bullet-shaped vesicular stomatitis virus.,Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH Science. 2010 Feb 5;327(5966):689-93. PMID:20133572[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
