4gkf
From Proteopedia
Contents |
Crystal structure and characterization of Cmr5 protein from Pyrococcus furiosus
Template:ABSTRACT PUBMED 23370277
Function
[CMR5_PYRFU] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage.
About this Structure
4gkf is a 2 chain structure with sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
Reference
- Park JH, Sun J, Park SY, Hwang HJ, Park MY, Shin M, Kim JS. Crystal structure of Cmr5 from Pyrococcus furiosus and its functional implications. FEBS Lett. 2013 Mar 18;587(6):562-8. doi: 10.1016/j.febslet.2013.01.029. Epub, 2013 Jan 28. PMID:23370277 doi:10.1016/j.febslet.2013.01.029
