Structural highlights
3tu4 is a 12 chain structure with sequence from Saccharomyces cerevisiae s288c and Xenopus laevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Gene: | hist1h2aj, LOC494591 (Xenopus laevis), SIR3, CMT1, MAR2, STE8, YLR442C, L9753.10 (Saccharomyces cerevisiae S288c) |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
Gene silencing is essential for regulating cell fate in eukaryotes. Altered chromatin architectures contribute to maintaining the silenced state in a variety of species. The silent information regulator (Sir) proteins regulate mating type in Saccharomyces cerevisiae. One of these proteins, Sir3, interacts directly with the nucleosome to help generate silenced domains. We determined the crystal structure of a complex of the yeast Sir3 BAH (bromo-associated homology) domain and the nucleosome core particle at 3.0 angstrom resolution. We see multiple molecular interactions between the protein surfaces of the nucleosome and the BAH domain that explain numerous genetic mutations. These interactions are accompanied by structural rearrangements in both the nucleosome and the BAH domain. The structure explains how covalent modifications on H4K16 and H3K79 regulate formation of a silencing complex that contains the nucleosome as a central component.
Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 A resolution.,Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE Science. 2011 Nov 18;334(6058):977-82. PMID:22096199[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE. Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 A resolution. Science. 2011 Nov 18;334(6058):977-82. PMID:22096199 doi:10.1126/science.1210915
