4jgc

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Template:STRUCTURE 4jgc

Contents 1 Human TDG N140A mutant IN A COMPLEX WITH 5-carboxylcytosine (5caC) 2 Function 3 About this Structure 4 Reference

Human TDG N140A mutant IN A COMPLEX WITH 5-carboxylcytosine (5caC)

Template:ABSTRACT PUBMED 23680598

Function

[TDG_HUMAN] In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

About this Structure

4jgc is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

• Hashimoto H, Zhang X, Cheng X. Activity and crystal structure of human thymine DNA glycosylase mutant N140A with 5-carboxylcytosine DNA at low pH. DNA Repair (Amst). 2013 May 13. pii: S1568-7864(13)00084-0. doi:, 10.1016/j.dnarep.2013.04.003. PMID:23680598 doi:10.1016/j.dnarep.2013.04.003