3r6w is a 2 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Azoreductases are well known for azo pro-drug activation by gut flora. We show that azoreductases have a wider role in drug metabolism than previously thought as they can also reduce and hence activate nitrofurazone. Nitrofurazone, a nitroaromatic drug, is a broad spectrum antibiotic which has until now been considered as activated in bacteria by nitroreductases. The structure of the azoreductase with nitrofurazone bound was solved at 2.08 A and shows nitrofurazone in an active conformation. Based on the structural information, the kinetics and stoichiometry of nitrofurazone reduction by azoreductase from P. aeruginosa, we propose a mechanism of activation which accounts for the ability of azoreductases to reduce both azo and nitroaromatic drugs. This mode of activation can explain the cytotoxic side-effects of nitrofurazone through human azoreductase homologues.
Activation of nitrofurazone by azoreductases: multiple activities in one enzyme.,Ryan A, Kaplan E, Laurieri N, Lowe E, Sim E Sci Rep. 2011;1:63. Epub 2011 Aug 12. PMID:22355582[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Ryan A, Kaplan E, Laurieri N, Lowe E, Sim E. Activation of nitrofurazone by azoreductases: multiple activities in one enzyme. Sci Rep. 2011;1:63. Epub 2011 Aug 12. PMID:22355582 doi:10.1038/srep00063
