1z4n is a 2 chain structure with sequence from Lactococcus lactis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase.,Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN. Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149 doi:http://dx.doi.org/10.1021/ja0509073
