An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts.,Schulze S, Mant A, Kossmann J, Lloyd JR FEBS Lett. 2004 May 7;565(1-3):101-5. PMID:15135060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Schulze S, Mant A, Kossmann J, Lloyd JR. Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts. FEBS Lett. 2004 May 7;565(1-3):101-5. PMID:15135060 doi:http://dx.doi.org/10.1016/j.febslet.2004.03.080
