Sequence alignment of human T-lymphotropic virus type I (HTLV-I) protease and other retroviral proteases reveals that the leukemia virus proteases contain residues at the C-terminus that are absent in the other proteases. We have prepared a mutant of HTLV-I protease that does not contain the 10 C-terminal residues and demonstrated that the catalytic efficiency of cleavage of a peptide substrate is unaffected.
The 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity.,Herger BE, Mariani VL, Dennison K, Shuker SB Biochem Biophys Res Commun. 2004 Aug 6;320(4):1306-8. PMID:15303275[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Herger BE, Mariani VL, Dennison K, Shuker SB. The 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1306-8. PMID:15303275
