Here we describe the crystal structure of modified [d(CGCGAATTCGCG)]2 refined to 2.04 A. The modification, which affects only the two thymines at the central ApT step, involves isosteric removal of the 2-keto oxygen atoms and substitution of the N1 nitrogen with carbon. The crystal structure reveals the ability of this modified thymine to effectively base pair with adenine in [d(CGCGAAtTCGCG)]2. The structure also suggests that the minor groove 'spine of hydration' is destabilized but essentially intact.
The role of minor groove functional groups in DNA hydration.,Woods KK, Lan T, McLaughlin LW, Williams LD Nucleic Acids Res. 2003 Mar 1;31(5):1536-40. PMID:12595562[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Woods KK, Lan T, McLaughlin LW, Williams LD. The role of minor groove functional groups in DNA hydration. Nucleic Acids Res. 2003 Mar 1;31(5):1536-40. PMID:12595562
