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1ldy
From Proteopedia
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HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND CYCLOHEXYL FORMAMIDE (CXF)
Overview
Amides are analogs of aldehydes and potent inhibitors of liver alcohol, dehydrogenases. They can be used for structural studies and for inhibiting, the metabolism of alcohols that form toxic products. We studied N-alkyl, amides that bind to the enzyme-NADH complex and act as uncompetitive, inhibitors against varied concentrations of ethanol (millimolar Kii, values, at pH 8 and 25 degrees C): N-propylacetamide (16), delta-valerolactam (1.6), N-formylpiperidine (0.14), N-isobutylformamide, (0.028), N-(cyclohexylmethyl)-formamide (0.011), and N-cyclohexylformamide, (0.0087). The lower affinity of delta-valerolactam and N-propylacetamide, can be explained by steric hindrance with Phe93 of the enzyme. Replacing, Phe93 with Ala in the S48T/F93A mutated enzyme, which resembles the, natural ... [(full description)]
About this Structure
1LDY is a [Single protein] structure of sequence from [Equus caballus] with ZN, NAD and CXF as [ligands]. Active as [Alcohol dehydrogenase], with EC number [1.1.1.1]. Structure known Active Site: NAD. Full crystallographic information is available from [OCA].
Reference
Binding of formamides to liver alcohol dehydrogenase., Ramaswamy S, Scholze M, Plapp BV, Biochemistry. 1997 Mar 25;36(12):3522-7. PMID:9132002
Page seeded by OCA on Tue Oct 30 15:42:16 2007
